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Summary Plants integrate environmental stimuli to optimize photosynthesis vs water loss by controlling stomatal apertures. However, stomatal responses to temperature elevation and the underlying molecular genetic mechanisms remain less studied.We developed an approach for clamping leaf‐to‐air vapor pressure difference (VPD_leaf) to fixed values, and recorded robust reversible warming‐induced stomatal opening in intact plants. We analyzed stomatal temperature responses of mutants impaired in guard cell signaling pathways for blue light, abscisic acid (ABA), CO₂, and the temperature‐sensitive proteins, Phytochrome B (phyB) and EARLY‐FLOWERING‐3 (ELF3).We confirmed thatphot1‐5/phot2‐1leaves lacking blue‐light photoreceptors showed partially reduced warming‐induced stomatal opening. Furthermore, ABA‐biosynthesis, phyB, and ELF3 were not essential for the stomatal warming response. Strikingly,Arabidopsis(dicot) andBrachypodium distachyon(monocot) mutants lacking guard cell CO₂sensors and signaling mechanisms, includinght1,mpk12/mpk4‐gc, andcbc1/cbc2abolished the stomatal warming response, suggesting a conserved mechanism across diverse plant lineages. Moreover, warming rapidly stimulated photosynthesis, resulting in a reduction in intercellular (CO₂). Interestingly, further enhancing heat stress caused stomatal opening uncoupled from photosynthesis.We provide genetic and physiological evidence that the stomatal warming response is triggered by increased CO₂assimilation and stomatal CO₂sensing. Additionally, increasing heat stress functions via a distinct photosynthesis‐uncoupled stomatal opening pathway.